Fig. 4: Spatiotemporal PA-Cre activation in free-moving mice with wireless LED.
From: Photoactivatable Cre knock-in mice for spatiotemporal control of genetic engineering in vivo
a A tiny wireless LED device and free-moving mice in which the LED was transplanted at the head and groin. Outline of the illumination experiments is also shown. b Macroscopic observation of the brain for the excitation of red fluorescent protein 2 weeks after LED implantation. Arrowhead: locus illuminated by blue LED. c Representative histology in each tissue with LED illumination. Scale bar: 200 µm. d Recombination efficiency of illuminated and nonilluminated sides in each tissue in F1 mice. Average data and standard deviations (error bars) were obtained from four pups. *p < 0.01. e Electrophoresis of the PCR products. White arrowhead: wild-type allele; black arrowhead: recombined allele; C: nonilluminated control locus; BL: locus illuminated with a blue LED device. f Enlarged images of the neurons expressing DsRed in the brain. Scale bar: 50 µm. g Projection of the neuronal pathway through the corpus callosum and pons from the cortex.
