Fig. 7:  NAC blocks the Asc-induced increase in whole-cell currents in etoposide-resistant WERI-Rb1 cells. | Laboratory Investigation

Fig. 7:  NAC blocks the Asc-induced increase in whole-cell currents in etoposide-resistant WERI-Rb1 cells.

From: Ascorbate-induced oxidative stress mediates TRP channel activation and cytotoxicity in human etoposide-sensitive and -resistant retinoblastoma cells

Fig. 7

a Time course recording of the currents increase induced by 1 mM Asc and currents decrease after application of 10 mM NAC. b Original traces of Asc-induced current responses to voltage ramps [current/voltage plot (I-V plot)]. Current densities are shown as a control without drugs (black trace labeled as A), during application of 1 mM Asc (green trace labeled as B) and after addition of 10 mM NAC (red trace labeled as C). Calculated current densities obtained by normalizing currents to membrane capacitance as function of imposed voltage were derived from the traces shown in (a). c Maximal negative inward current amplitudes induced by a voltage step from 0 mV to −60 mV, shown in percent of control values before application of drugs (control set to 100%). The asterisks (*) designate a significant increase in inward currents after application of 1 mM Asc and a significant suppression of inward currents after addition of 10 mM NAC (paired tested). d Same diagram but related to maximal outward current amplitudes induced by a voltage step from 0 mV to +130 mV. Asc Ascorbic acid, NAC N-acetylcysteine.

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