Table 1 List of oligonucleotides used for TRP-cation channel mRNA expression analyses in human etoposide-sensitive and -resistant WERI-Rb1 by qRT-PCR.
Gene | Oligonucleotide sequence | Product size (bp) | Oligonucleotide efficiency | GenBank accession number |
|---|---|---|---|---|
TRPA1_for | TGGACACCTTCTTCTTGCATT | 103 | 1.0 | NM_007332 |
TRPA1_rev | TCATCCATTTCATGCAGCAC | |||
TRPC5_for | TGGTAACTGGTTCAACAACACC | 99 | 1.0 | NM_012471 |
TRPC5_rev | CTGTCAGCATTGCGTTCTG | |||
RPS18_for | CTTCCACAGGAGGCCTACAC | 82 | 1.0 | NM_022551 |
RPS18_rev | CGCAAAATATGCTGGAACTTT | |||
TRPM2_for | CGAGGACATCAGCAATAAGGT | 75 | 0.836 | NM_003307 |
TRPM2_rev | ATGGAGCCCGACCTCTTC | |||
TRPM7_for | TTGACATTGCCAAAAATCATGT | 66 | 1.0 | NM_017672 |
TRPM7_rev | CTTGTTCCAAGGATCCAACC | |||
TRPM8_for | GGTCCTGTACTCGCTGGTCT | 67 | 1.0 | NM_024080 |
TRPM8_rev | CACCCCATTTACGTACCACTG | |||
TRPV1_for | CTACAGCAGCAGCGAGACC | 71 | 0.899 | NM_080704 |
TRPV1_rev | CCTGCAGGAGTCGGTTCA | |||
TRPV4_for | CAACAACGACGGCCTCTC | 74 | 1.0 | NM_021625 |
TRPV4_rev | GGATGATGTGCTGAAAGATCC |
