Fig. 5: GTSE1 promotes prostate cancer proliferation via the SP1/FOXM1 signaling pathway.

A GTSE1, FOXO1, FOXO3a, FOXO4, and FOXM1 were detected by western blotting. B CCNB1 and CCND1 could be positively regulated by GTSE1. C The efficiency of shFOXM1 and thiostrepton was measured. D Cell viability was measured by a Cell Counting Kit-8 assay. E Colony formation by LNCaP cells treated with or without shFOXM1 or Thiostrepton was evaluated. The left side contains representative pictures, and the right side shows the quantized results. F LNCaP cells treated with or without shFOXM1 or Thiostrepton (left image) were evaluated with an EdU assay. Proliferation rates are also shown (right image). G Based on the FIREBROWSE database, GTSE1 was positively correlated with FOXM1 at the mRNA level. H qPCR confirmed that GTSE1 positively regulates the transcription of FOXM1. I GTSE1, HIF-1α, SP1 and E2F1 were detected by western blotting. J SP1 was identified as a critical factor participating in the regulatory effect of GTSE1 on FOXM1.