Fig. 2: 3D multiphoton microscopy of unlabeled nephrectomy. | Laboratory Investigation

Fig. 2: 3D multiphoton microscopy of unlabeled nephrectomy.

From: Large-scale, three-dimensional tissue cytometry of the human kidney: a complete and accessible pipeline

Fig. 2

Mosaic of high-resolution image volumes collected from a 4 mm by 9 mm, 50 μm thick section of paraformaldehyde-fixed human nephrectomy tissue. A Maximum projection image of 3D volume of tissue autofluorescence. B Maximum projection of 3D volume of second-harmonic generation (SHG) images. C Overlay of autofluorescence and SHG. D–F Show corresponding ×4 magnification images of the region indicated in the upper box in (A), and (G–I) show corresponding ×4 magnification images of the region indicated in the lower box in (A). Arrows in (G, H) indicate regions of apparent tubular drop out and fibrosis. Scale bar in (A) represents 1 mm. Scale bar in (D) represents 250 μm in (D–I).

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