Fig. 3: 3D confocal immunofluorescence of structural markers. | Laboratory Investigation

Fig. 3: 3D confocal immunofluorescence of structural markers.

From: Large-scale, three-dimensional tissue cytometry of the human kidney: a complete and accessible pipeline

Fig. 3

A Maximum projection of combined autofluorescence and SHG images. B Maximum projection of mosaic of confocal fluorescence image volumes of Oregon-Green phalloidin. C Overlay of maximum projection confocal fluorescence images of phalloidin (green) and antibodies to Tamm–Horsfall Protein (THP, cyan) and aquaporin-1 (AQP1, magenta). D–F Show corresponding ×4 magnification images of the region indicated in the upper box in (A), and (G–I) show corresponding ×4 magnification images of the region indicated in the lower box in (A). Arrows in (G–I) indicate regions of fibrosis and apparent tubular drop out. Scale bar in (A) represents 1 mm. Scale bar in (D) represents 250 μm.

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