Fig. 5: 3D confocal fluorescence and multiphoton autofluorescence/SHG microscopy of regions of apparent injury. | Laboratory Investigation

Fig. 5: 3D confocal fluorescence and multiphoton autofluorescence/SHG microscopy of regions of apparent injury.

From: Large-scale, three-dimensional tissue cytometry of the human kidney: a complete and accessible pipeline

Fig. 5

A Maximum projection of combined 3D fluorescence image volume of phalloidin (green) and antibodies to THP (cyan), AQP1 (magenta), myeloperoxidase (MPO, red), CD68 (yellow), and CD3 (white). B–E ×4 magnifications of regions indicated in boxes in (A). Top row—confocal fluorescence images. Middle row—corresponding autofluorescence images. Bottom row—corresponding SHG images. Scale bar in (A) represents 1 mm and in (B) 250 μm for (B–E).

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