Fig. 4: SNHG5 regulates the expression of CTGF mediated by miR-26b. | Laboratory Investigation

Fig. 4: SNHG5 regulates the expression of CTGF mediated by miR-26b.

From: LncRNA SNHG5 upregulation induced by YY1 contributes to angiogenesis via miR-26b/CTGF/VEGFA axis in acute myelogenous leukemia

Fig. 4

a The expression of CTGF was detected in AML cells using qRT-PCR and western blot. b The mRNA and protein levels of CTGF were detected in AML cells transfected with miR-26b or negative control. c Bioinformatics analysis showed the prediction for miR-26b bindings sites on the 3′-UTR of CTGF. d Luciferase activity in AML cells overexpressing of miR-26b and co-transfection of luciferase reporters containing SNHG5 3′-UTR or mutant. e The expression of CTGF in AML cells upon treatment of sh-SNHG5 with miR-26b inhibitor or negative control was detected using qRT-PCR and western blot, respectively. “**” denotes p < 0.01; “***” denotes p < 0.001. Data are expressed as the mean ± SD.

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