Fig. 6: YY1 activates the transcription of lncRNA SNHG5. | Laboratory Investigation

Fig. 6: YY1 activates the transcription of lncRNA SNHG5.

From: LncRNA SNHG5 upregulation induced by YY1 contributes to angiogenesis via miR-26b/CTGF/VEGFA axis in acute myelogenous leukemia

Fig. 6

a YY1 expression in AML cells assessed with qRT-PCR analysis. b The expression of YY1 and SNHG5 in cells transfected with sh-YY1 or shRNA control as well as pcDNA3.1-YY1 or pcDNA3.1-NC. c The binding motif of transcription factor YY1 from JASPAR database. d The two potential putative binding sites upstream of SNHG5 named P1 and P2 were predicted. e The enrichment of YY1 on the promoter of SNHG5 was assessed with ChIP assay. f Luciferase activity in AML cells overexpressing of YY1 and co-transfection of luciferase reporters containing SNHG5. “*” denotes p < 0.05; “**” denotes p < 0.01; “***” denotes p < 0.001. Data are expressed as the mean ± SD.

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