Fig. 5: Increased monocyte adhesion to the endothelium in response to hemoglobin-derived peptides and ferryl hemoglobin. | Laboratory Investigation

Fig. 5: Increased monocyte adhesion to the endothelium in response to hemoglobin-derived peptides and ferryl hemoglobin.

From: Hemoglobin oxidation generates globin-derived peptides in atherosclerotic lesions and intraventricular hemorrhage of the brain, provoking endothelial dysfunction

Fig. 5

Adhesion of monocytes to HUVECs after exposure to FHb (10 µmol/L) and peptides (P1–P5) (10 µmol/L). HUVECs were stained for DNA (Hoechst 33258, blue) and F-actin (cytoskeleton, red, iFluor 647). Monocytes were stained with calcein-AM. The mean fluorescence intensity is shown (a). Magnified images demonstrating monocyte adhesion to endothelial cells after Peptide 3 treatment (B). Representative images, n = 5. The results are shown as the mean values ± SEMs of the experiments. *P < 0.05, **P < 0.01, ***P < 0.001. CTR means untreated.

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