Fig. 1: Assessment of liver injury in WT and FXRInt−/− mice fed control or chronic (the Lieber-DeCarli diet containing 5% ethanol)-binge ethanol.
From: Enhanced alcoholic liver disease in mice with intestine-specific farnesoid X receptor deficiency
a Activities of serum ALT, AST, total bilirubin (T. Billi), ALP, and serum levels of TG, TC, and total BAs; b H&E staining of WT and FXR Int−/− mice. Top figures no EtOH and bottom micrographs show ethanol-induced changes including microvesicular steatosis (open arrow) in WT mice, macrovesicular steatosis (arrow) in FXRInt−/− mice, hepatocyte ballooning (open triangle), and MDBs (black arrow); c hepatic levels of TG and TC; d hepatic staining of TUNNEL. Values are presented as mean ± SD (n = 11, 15, 10, and 15 mice/group for WT/Veh, WT/EtOH, FXRInt−/−/Veh, and FXRInt−/−/EtOH, respectively). An asterisk (*) indicates statistical significance (p < 0.05) of ethanol treatment (EtOH) compared with vehicle (Veh) control group in the same strain. A hash symbol (#) indicates statistical significance between WT and FXRInt−/− mice (p < 0.05) with the same treatment. The scale bars show 50 μm for ×200 magnification.
