Fig. 5: Effects of ethanol feeding on the intestinal mucus layer and villi morphology.
From: Enhanced alcoholic liver disease in mice with intestine-specific farnesoid X receptor deficiency
a Representative images of H&E staining of the distal ileum. The ileum of vehicle-fed FXRInt−/− mice showed distorted mucosal lining with breaches along the mucosal lining. The shortening and opening of crypts to the lumen were observed in ethanol-fed mice (black arrow); b PAS staining of the intestine. Mucus were produced in villi (open triangle), and were detected in the intestinal lumen between villi (star) after ethanol fed. For neutral glycoproteins (magenta), mucins that are both acidic and neutrally charged (purple) and acidic glycoproteins (blue); c MUC2 IHC of the small intestine (black triangle) demonstrated release of MUC2 from goblet cells in the villi following alcohol consumption (star). The scale bars show 50 μm for ×200 magnification.
