Fig. 2: Design of the study. | Laboratory Investigation

Fig. 2: Design of the study.

From: An improved assay for detection of theranostic gene translocations and MET exon 14 skipping in thoracic oncology

Fig. 2

New lung cancers eligible for genotyping (non-small cell non-squamous lung cancer or occurring in a never or light smoker) referred to the molecular platform were tested in parallel by the routine process (i.e., genotyping by high-throughput analysis or SNaPshot multiplex kit, ALK and ROS1 IHC) and the LD-RT-PCR assay. Whenever ALK or ROS1 IHC was positive, a break-apart FISH was performed. If results of the routine process and those of LD-RT-PCR were discordant, further molecular techniques were performed. FFPE formalin-fixed paraffin-embedded, IHC immunohistochemistry. LD-RT-PCR ligation-dependent reverse transcription polymerase chain reaction.

Back to article page