Fig. 5: Macrophage polarization in wild-type mice and PD-1−/− mice after skeletal muscle contusion. | Laboratory Investigation

Fig. 5: Macrophage polarization in wild-type mice and PD-1−/− mice after skeletal muscle contusion.

From: Programmed death-1 promotes contused skeletal muscle regeneration by regulating Treg cells and macrophages

Fig. 5

a iNOS immunofluorescence staining of pro-inflammatory macrophage markers. b Arg1 immunofluorescence staining of anti-inflammatory macrophage markers. c Average pro-inflammatory macrophages in a single field of view. d Average anti-inflammatory macrophages in a single field of view. e Mac-2 mRNA expression at various time points after skeletal muscle contusion. f iNOS mRNA expression at various time points after skeletal muscle contusion. g CD86 mRNA expression at various time points after skeletal muscle contusion. h Arg1 mRNA expression at various time points after skeletal muscle contusion. i CD206 mRNA expression at various time points after skeletal muscle contusion. j CD163 mRNA expression at various time points after skeletal muscle contusion. Data are mean ± SD, n (WT) = 8, n (PD-1−/−) = 6. aCompared with WTcon group or WT1 group, P < 0.05; bCompared with Pcon group or P1 group, P < 0.05; cat the same time point, the PD-1−/− mice compared with wild-type mice, P < 0.05.

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