Fig. 2: Elevated expression of SLCO4A1 is detected in CD133+CD44+ cells.

A Expression of tumor stem cell maker CD133+CD44+ in human colon cancer HCT116 cells detected by flow cytometry. B Expression of SLCO4A1 in colon epithelial cells NCM460, HCT116 and CD133+CD44+ cells determined by RT-qPCR, expression of SLCO4A1 normalized to GAPDH, *p < 0.05 vs. colon epithelial cells NCM460, **p < 0.01 vs. HCT116 cells. The data were the measurement data and expressed as mean ± standard deviation. One-way ANOVA was used for the data analysis among multiple groups, followed by Tukey’s post hoc test. The cell experiment was repeated three times.