Fig. 1: miR-182 was downregulated in the spinal cords of SCI mice. | Laboratory Investigation

Fig. 1: miR-182 was downregulated in the spinal cords of SCI mice.

From: MicroRNA-182 improves spinal cord injury in mice by modulating apoptosis and the inflammatory response via IKKβ/NF-κB

Fig. 1

A The BBB scores at 1, 3, 7, 14, 21, and 28 days after SCI were shown for all groups of mice (n = 6/group). B Quantification of spared tissue within the injury site, and 1600 μm rostral and caudal to the epicenter, 28 days post injury (n = 6/group). C Spinal cord water content was assessed using wet-to-dry weight method (n = 6/group). D Apoptosis was determined by TUNEL staining at 1 and 7 days post injury (n = 6/group). E, F The expression of casapse-3 was measured by IHC at 1 and 7 days after SCI (×200 magnification) (n = 6/group). G A cluster heap map was used to present the upregulated and downregulated miRNAs from GSE19890. H qRT-PCR was performed to detect the expression of miR-182, miR-124, miR-126, miR-21, and miR-223 (n = 6/group). I qRT-PCR was performed to determine the expression levels of miR-182 in spinal cord tissues from mice at 1, 7, 14, 21, and 28 days after SCI (n = 6/group/time). Data represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01 vs. Sham group.

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