Fig. 3: miR-182 inhibited the inflammatory response in SCI mice. | Laboratory Investigation

Fig. 3: miR-182 inhibited the inflammatory response in SCI mice.

From: MicroRNA-182 improves spinal cord injury in mice by modulating apoptosis and the inflammatory response via IKKβ/NF-κB

Fig. 3

The mice were subjected to SCI and treated intrathecally with agomir-miR-182/agomir-NC (2 μl, 20 µM). At 7 days post injury, the animals were sacrificed following pentobarbital (0.3 g/kg, i.p) anesthesia, and subsequently, the spinal cord tissues were harvested for further experiments. A–D ELISA analysis of TNF-α, IL-6, IL-10, and IL-1β in serum of SCI rat after agomir-miR-182 injection (n = 6/group). E Western blot analysis of TNF-α, IL-6, IL-10, and IL-1β in spinal cord tissues (n = 6/group). Data represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01 vs. Sham group; ##p < 0.01 vs. SCI + agomir-NC group.

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