Fig. 4: Overexpression of miR-182 suppressed the inflammatory response and apoptosis in an SCI cell culture model. | Laboratory Investigation

Fig. 4: Overexpression of miR-182 suppressed the inflammatory response and apoptosis in an SCI cell culture model.

From: MicroRNA-182 improves spinal cord injury in mice by modulating apoptosis and the inflammatory response via IKKβ/NF-κB

Fig. 4

A Agomir-miR-182 was added to the cultured BV-2 cells (1 × 106/well) and incubated for 24 h, and then the transfected efficiency of miR-182 was detected by qRT-PCR analysis. B BV-2 cells (1 × 106/well) were treated with different concentrations of LPS (10, 100, and 1000 ng/ml) for 24 h, and the expression of miR-182 was detected by qRT-PCR analysis. Agomir-miR-182 was added to the cultured BV-2 cells (1 × 106/well) 4 h prior to LPS treatment and incubated for 24 h, and then cells were harvested for subsequent experiments. C Activity of caspase-3 was measured using a commercial kit. D The protein expression level of caspase-3 was detected by IFA. E The protein expression levels of Bcl-2, Bax, cleaved-caspase-3, and cleaved-PARP were detected by western blot analysis. F–I The expression of TNF-α, IL-6, IL-10, and IL-1β were measured by ELISA analysis. Data represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01 vs. Control group; ##p < 0.01 vs. LPS + agomir-NC group.

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