Fig. 5: IKKβ is a direct target of miR-182 in BV-2 cells. | Laboratory Investigation

Fig. 5: IKKβ is a direct target of miR-182 in BV-2 cells.

From: MicroRNA-182 improves spinal cord injury in mice by modulating apoptosis and the inflammatory response via IKKβ/NF-κB

Fig. 5

A The predicted miR-182 binding sites on IKKβ. B Luciferase assay of BV-2 cells (1 × 106/well) co-transfected with firefly luciferase constructs containing the IKKβ wild-type or mutated 3′-UTRs and agomir-miR-182 or agomir-NC, as indicated (n = 3). Data represent the mean ± SD of three independent experiments. **p < 0.01 vs. agomir-NC group. C The protein levels of IKKβ were detected by western blot after agomir-miR-182 transfection. Agomir-miR-182 and pcDNA-IKKβ were co-transfected into the cultured BV-2 cells 4 h prior to LPS treatment and incubated for 24 h. D The NF-κB signaling pathway activity in BV-2 cells was measured by a Promega luciferase assay kit. E The protein expression levels of IKKβ, p-IκBα, IκBα, nuclear-p-p65 and total p65 were detected by western blot analysis. Data represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01 vs. Control group; ##p < 0.01 vs. LPS + agomir-NC group.

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