Fig. 7: miR-182 blocked the activation of the NF-κB pathway in vivo. | Laboratory Investigation

Fig. 7: miR-182 blocked the activation of the NF-κB pathway in vivo.

From: MicroRNA-182 improves spinal cord injury in mice by modulating apoptosis and the inflammatory response via IKKβ/NF-κB

Fig. 7

The mice were subjected to SCI and treated intrathecally with agomir-miR-182/agomir-NC (2 μl, 20 µM). At 7 days post injury, the animals were sacrificed following pentobarbital sodium (50 mg/kg, i.p) anesthesia, and subsequently, the spinal cord tissues were harvested for further experiments. A The protein expression levels of IKKβ, p-IκBα, IκBα, nuclear-p-p65, and total p65 were detected by western blot analysis (n = 6/group). B The bands were semi-quantitatively analyzed by using Image J software, normalized to β-actin density. C The expression of IKKβ, p-IκBα, and nuclear-p-p65 was detected by IHC. Data represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01 vs. Sham group; ##p < 0.01 vs. SCI + agomir-NC group.

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