Fig. 3: miR-27a was a downstream target of NEAT1.
From: lncRNA NEAT1 aggravates sepsis-induced lung injury by regulating the miR-27a/PTEN axis
A StarBase software was used to predict the potential binding sites between miR-27a and NEAT1. B Luc-WT-NEAT1 or Luc-MUT-NEAT1 plasmids were co-transfected with miR-27a mimics, miR-27a inhibitor or their negative controls in 16HBE cells, and luciferase activity was measured. C, D 16HBE and BEAS-2B cells were transfected with pcDNA-NC, pcDNA-NEAT1, sh-NC or sh-NEAT1 for 24 h, and the expression levels of NEAT1 and miR-27a were detected using qPCR. E Plasma samples were collected from patients with ARDS and controls, and the expression level of miR-27a was detected using qPCR. F Pearson correlation scatter plot showed that miR-27a expression was inversely correlated with NEAT1 expression. Data are presented as the means ± SD and were analysed using Student’s t test or one-way analysis of variance. *P < 0.05, **P < 0.01, and ***P < 0.001.
