Fig. 5: PTEN functioned as an effector of miR-27a.

A Plasma samples were collected from patients with ARDS and controls, and the expression of PTEN was detected using qPCR. B Pearson correlation scatter plot shows that miR-27a expression was inversely correlated with PTEN expression. C The potential binding site between miR-27a and PTEN. D Luc-WT-PTEN or Luc-MUT-PTEN plasmids were co-transfected with miR-27a mimics, miR-27a inhibitor or their negative controls in 16HBE cells, and luciferase activity was measured. E, F 16HBE and BEAS-2B cells were transfected with miR-27a mimics, miR-27a inhibitor or their negative control for 24 h. The expression levels of miR-27a and PTEN were detected using qPCR. G 16HBE and BEAS-2B cells were transfected with miR-27a mimic alone or co-transfected with miR-27a mimics and sh-PTEN. The protein levels of PTEN, p21, CDK2 and Cyclin D1 were detected using western blot. Data are presented as the means ± SD and were analysed using Student’s t test or one-way analysis of variance. *P < 0.05, **P < 0.01, and ***P < 0.001.