Fig. 7: The role of NEAT1 knockdown in CLP-induced lung injury in mice.
From: lncRNA NEAT1 aggravates sepsis-induced lung injury by regulating the miR-27a/PTEN axis
CLP surgery was performed on the fifth day after lentivirus injection, and lung samples were obtained immediately after the mice were euthanized. A–C The expression levels of NEAT1, miR-27a and PTEN in lung tissues from CLP-induced septic mice were analysed using qPCR. D The effect of NEAT1 knockdown on the survival rate of CLP-induced septic mice. E The levels of TNF-α, IL-6 and IL-1β in lung tissues of CLP-induced septic mice were detected using ELISA. F The protein levels of p21, CDK2 and Cyclin D1 in lung tissues from CLP-induced septic mice were detected using western blot. Data are presented as the means ± SD and were analysed using Student’s t test or one-way analysis of variance. *P < 0.05, **P < 0.01, and ***P < 0.001.
