Fig. 2: Knockdown of MALAT1 represses angiogenesis in vitro. | Laboratory Investigation

Fig. 2: Knockdown of MALAT1 represses angiogenesis in vitro.

From: Long non-coding RNA MALAT1 enhances angiogenesis during bone regeneration by regulating the miR-494/SP1 axis

Fig. 2

A MC3T3-E1 cells were treated with osteogenic medium for 5, 10, or 15 days. VEGFA concentration in the supernatant was evaluated by ELISA. B The VEGFA concentration of MC3T3-E1 cells transfected with or without MALAT1 shRNA in the supernatant was evaluated by ELISA. C–E HUVECs were treated with the supernatant from MC3T3-E1 cells transfected with MALAT1 knockdown or control shRNA. C Proliferation of HUVECs was detected using the MTT assay. D Migration of HUVECs was measured by Transwell assay. E Tube formation of HUVECs was observed under a microscope, and an image of each well is shown. Data are the mean ± SD for three separate experiments. The P value was determined by ANOVA following Tukey’s post hoc test or Student’s t-test. *P < 0.05, **P < 0.01, and ***P < 0.001.

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