Fig. 3: MALAT1 mediates the SP1/TLR2/BMP2 axis by targeting miR-494. | Laboratory Investigation

Fig. 3: MALAT1 mediates the SP1/TLR2/BMP2 axis by targeting miR-494.

From: Long non-coding RNA MALAT1 enhances angiogenesis during bone regeneration by regulating the miR-494/SP1 axis

Fig. 3

A Potential binding sites of miR-494 and MALAT1, miR-494 and SP1 were predicted using bioinformatics methods. B The luciferase activity was examined using a dual luciferase reporter assay. C Expression levels of miR-494 in MC3T3-E1 cells transfected with or without MALAT1 shRNA were detected by qRT-PCR. D mRNA expression of SP1 was assessed by qRT-PCR. E Protein expression of SP1, TLR2, and BMP2 was determined by western blot assay. Data are the mean ± SD of three separate experiments. The P value was determined by ANOVA following Tukey’s post hoc test or Student’s t-test. *P < 0.05, **P < 0.01, and ***P < 0.001.

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