Fig. 4: MiR-494 overexpression inhibits angiogenesis and osteogenic differentiation. | Laboratory Investigation

Fig. 4: MiR-494 overexpression inhibits angiogenesis and osteogenic differentiation.

From: Long non-coding RNA MALAT1 enhances angiogenesis during bone regeneration by regulating the miR-494/SP1 axis

Fig. 4

A Expression levels of miR-494 were evaluated by qRT-PCR. B–E MC3T3-E1 cells were transfected with miR-494 mimics or NC mimics. B Activity of ALP was detected using an ALP detection kit. C ARS assay was performed to assess mineralized nodules. D The protein expression of RUNX2 and OSX was determined by western blotting. E VEGFA concentrations in the supernatant were determined by ELISA. F–H HUVECs were treated with the supernatant from MC3T3-E1 cells transfected with miR-494 mimics or NC mimics. F Proliferation of HUVECs was detected by MTT assay. G Migration of HUVECs was measured using Transwell assays. H Tube size and numbers of HUVECs were observed under a microscope and imaged. Data are the mean ± SD for three separate experiments. The P value was determined by ANOVA following Tukey’s post hoc test and Student’s t-test. *P < 0.05, **P < 0.01, and ***P < 0.001.

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