Fig. 5: SP1 overexpression or miR-494 knockdown attenuates the inhibition of sh-MALAT1 on angiogenesis and osteogenic differentiation. | Laboratory Investigation

Fig. 5: SP1 overexpression or miR-494 knockdown attenuates the inhibition of sh-MALAT1 on angiogenesis and osteogenic differentiation.

From: Long non-coding RNA MALAT1 enhances angiogenesis during bone regeneration by regulating the miR-494/SP1 axis

Fig. 5

MC3T3-E1 cells were cotransfected with MALAT1 shRNA and miR-494 inhibition or SP1 overexpression plasmid. A The activity of ALP was detected using an ALP detection kit. B An ARS assay was performed to assess mineralized nodules. C Protein expression of SP1, TLR2, BMP2, RUNX2, and OSX was determined by western blotting. D The VEGFA concentrations in the supernatant were evaluated by ELISA. E–G HUVECs were treated with the supernatant from MC3T3-E1 cells. E Proliferation of HUVECs was detected by MTT assay. F Migration of HUVECs was measured by Transwell assays. G Tube formation of HUVECs in each well was observed under a microscope and then imaged. Data are the mean ± SD for three separate experiments. The P value was determined by ANOVA following Tukey’s post hoc test or Student’s t-test. *P < 0.05, **P < 0.01, and ***P < 0.001.

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