Fig. 4: Validation cohort.
From: Establishing standardized immune phenotyping of metastatic melanoma by digital pathology
Linear regression and correlation analysis between the digitally assessed and classified immune diagnoses and the pathologists’ diagnoses in the validation cohort (A). While all cases from the diagnoses “desert” and “inflamed” grouped to their appropriate category, three cases from the diagnoses “excluded” clustered to “inflamed” (red arrow) when applying the suggested algorithm. The black line shows the measured linear regression line with the 95% confidence interval, the green line depicts the perfect fit (A). Densities of CD8+ T cells in the validation cohort according to their spatial distribution among the tumor compartments intratumoral (iCD8+ T cells; left; dark gray columns), stroma (sCD8+ T cells; middle; light gray columns), and invasive margin (imCD8+ T cells; right; white columns) in relation to the digital-based immune diagnosis and site of metastasis (B–D); line at 0.06 CD8+ T cells/μm2 (Color figure online).
