Fig. 4: siRNA-mediated knockdown of PLCG1 arrested the cell cycle and promoted apoptosis. | Laboratory Investigation

Fig. 4: siRNA-mediated knockdown of PLCG1 arrested the cell cycle and promoted apoptosis.

From: Phospholipase Cγ1 (PLCG1) overexpression is associated with tumor growth and poor survival in IDH wild-type lower-grade gliomas in adult patients

Fig. 4

a Representative graphs showing cell cycle distribution. b The statistical results of the cell cycle distribution (G1, G2, and S) of SW1088 (up) and SW1783 (down) cells in the control and si1303 groups. c Representative graphs showing the effect of U73122 on early and late apoptosis by annexin V–PI flow cytometry assays. d The statistical results of the SW1088 (up) and SW1783 cell (down) apoptosis assays. e The Western blot verified the flow cytometry results of cell cycle and apoptosis. f The statistical results of the Western blot in the control and si1303 groups of CDK1 and Caspase3. ***p < 0.001, **p < 0.01, *p < 0.05. The data are expressed as the means ± SD.

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