Fig. 4: Changes in free and high-fat BRL 3 A cells.

a cell viability tested by CCK-8; b–d metabolic enzyme abundance of Acc, Fasn, and Ldlr; e–g the abundance of Sirt6, Ampk, and mtor mRNA; h–m: levels of SIRT6, p-AMPKα/AMPKα, p-mTOR/mTOR, p-Raptor/Raptor, and Rheb in BRL 3A cells. *Compared with the NC group, P < 0.05; #Compared with the ST group, P < 0.05. n = 6 per group.