Table 1 Information of reagents, primers and antibodies.
Names | Information |
|---|---|
For treatment | |
Sirt6 siRNA forward sequence | 5′ - GCC AGA AUG UAG ACG GGC UTT - 3′ for rat, GenePharma |
Sirt6 siRNA reverse sequence | 5′ - AGC CCG UCU ACA UUC UGG CTT - 3′ for rat, GenePharma |
Negative control siRNA forward sequence | 5′ - UUG UCC GAA CGU GUC ACG UTT - 3′ for rat, GenePharma |
Negative control siRNA reverse sequence | 5′ - ACG UGA CAC GUU CGG AGA ATT - 3′ for rat, GenePharma |
SIRT6 | recombinant human SIRT6 protein, ab104030, Abcam |
AICAR | AMPK agonist, #9944, Cell Signaling Technology |
Rapamycin | mTOR inhibitor, #9904, Cell Signaling Technology |
T0901317 | LXR agonist, ab142808, Abcam |
For detection | |
Sirt6 primer forward sequence | 5′ - GGA TGG ACC CCG AGT GCT - 3′ for rat, GenePharma |
Sirt6 primer reverse sequence | 5′ - GGG CTT GGG CTT ATA GGA A - 3′ for rat, GenePharma |
Ampk primer forward sequence | 5′ - GCT CGC AGT GGC TTA TCA T - 3′ for rat, GenePharma |
Ampk primer reverse sequence | 5′ - TGG ACA GCG TGC TTT GG - 3′ for rat, GenePharma |
mtor primer forward sequence | 5′ - GGC TTC TGA AGA TGC TGT CC - 3′ for rat, GenePharma |
mtor primer reverse sequence | 5′ - GAG TTC GAA GGG CAA GAG TG - 3′ for rat, GenePharma |
Srebp1c primer forward sequence | 5′ - GGA GCC ATG GAT TGC ACA TT - 3′ for rat, GenePharma |
Srebp1c primer reverse sequence | 5′ - AGG AAG GCT TCC AGA GAG GA - 3′ for rat, GenePharma |
Acc primer forward sequence | 5′ - AAC ATC CCG CAC CTT CTT CTA C - 3′ for rat, GenePharma |
Acc primer reverse sequence | 5′ - CTT CCA CAA ACC AGC GTC TC- 3′ for rat, GenePharma |
Fasn primer forward sequence | 5′ - CAG GTG TGT GAT GGG AAG - 3′ for rat, GenePharma |
Fasn primer reverse sequence | 5′ - TGT GGA TGA TGT TGA TGA TAG- 3′ for rat, GenePharma |
Ldlr primer forward sequence | 5′ - GTG GCA GTA GTG AGT GTA TC - 3′ for rat, GenePharma |
Ldlr primer reverse sequence | 5′ - ATC CCA AAG ACG AGA AGT- 3′ for rat, GenePharma |
Gck primer forward sequence | 5′ - CAG TGG AGC GTG AAG ACA AA- 3′ for rat, GenePharma |
Gck primer reverse sequence | 5′ - TTG GTC CAA TTG AGG AGG A- 3′ for rat, GenePharma |
Pck1 primer forward sequence | 5′ - AGT TCG TGG AAG GCA ATG CT - 3′ for rat, GenePharma |
Pck1 primer reverse sequence | 5′ - CTT CAG CTT GCG GAT GAC AC- 3′ for rat, GenePharma |
Bact primer forward sequence | 5′ - TGA CAG GAT GCA GAA GGA GAT TAC - 3′ for rat, GenePharma |
Bact primer reverse sequence | 5′ - GAG CCA CCA ATC CAC ACA GA - 3′ for rat, GenePharma |
SIRT6 primary antibody | rabbit anti-rat, approximately 42 kDa (predicted 39 kDa), ab191385, 1:2000 for WB and 1:1000 IF, Abcam |
AMPKα primary antibody | rabbit anti-rat, 62 kDa, #5831, for WB, Cell Signaling Technology |
p-AMPKα primary antibody (Thr172) | rabbit anti-rat, 62 kDa, #2535, for WB, Cell Signaling Technology |
mTOR primary antibody | rabbit anti-rat, 289 kDa, #2983, for WB, Cell Signaling Technology |
p-mTOR primary antibody (Ser2448) | rabbit anti-rat, 289 kDa, #5536, for WB, Cell Signaling Technology |
Raptor primary antibody | rabbit anti-rat, 150 kDa, #2280, for WB, Cell Signaling Technology |
p-Raptor primary antibody (Ser792) | rabbit anti-rat, 150 kDa, #2083, for WB, Cell Signaling Technology |
Rheb primary antibody (Ser792) | rabbit anti-rat, 16 kDa, #13879, for WB, Cell Signaling Technology |
SREBP1c primary antibody | mouse anti-rat, 68/125 kDa, sc-365513, for WB and IF, Santa Cruz Biotechnology |
SCAP primary antibody | rabbit anti-rat, 130 kDa, #13102, for WB, Cell Signaling Technology |
Insig1 primary antibody | rabbit anti-rat, 30/50 kDa, ab70784, for WB, Abcam |
Insig2 primary antibody | rabbit anti-rat, 25 kDa, ab86415, for WB, Abcam |
Lipin-1 primary antibody | rabbit anti-rat, 99/110 kDa, ab70138, for WB, Abcam |
LXRα primary antibody | rabbit anti-rat, 50 kDa, #ab176323, for WB, Abcam |
β-actin primary antibody | rabbit anti-rat, 45 kDa, #4970, for WB, Cell Signaling Technology |
Alexa Fluor® 488 Conjugate | anti-mouse IgG (H + L), F(ab’)2 Fragment, #4408, for IF, Cell Signaling Technology |
Alexa Fluor® 555 Conjugate | anti-rabbit IgG (H + L), F(ab’)2 Fragment, #4413, for IF, Cell Signaling Technology |
DAPI | for nucleus, #4083, for IF, Cell Signaling Technology |
Anti-rabbit IgG-HRP secondary antibody | anti-rabbit, #7074, for WB, Cell Signaling Technology |
Anti-mouse IgG-HRP secondary antibody | anti-mouse, #7076, for WB, Cell Signaling Technology |
