Fig. 2: Combined RNA in situ hybridization for SLUG and multiple immunohistochemistry for CK19 and P63 in normal parotid glands. | Laboratory Investigation

Fig. 2: Combined RNA in situ hybridization for SLUG and multiple immunohistochemistry for CK19 and P63 in normal parotid glands.

From: SLUG is a key regulator of epithelial-mesenchymal transition in pleomorphic adenoma

Fig. 2

A Representative hematoxylin and eosin image of a parotid gland. B In situ hybridization for SLUG (white dots) and multiplex immunohistochemistry for CK19 (green cytoplasmic stain) and P63 (red nuclear stain) were performed on a single slide. The white and red boxed areas are enlarged in (C and D), respectively. C P63-positive myoepithelial cells around striated and intercalated ducts expressed SLUG (red arrows). D Myoepithelial cells surrounding acini were also positive for SLUG (yellow arrows). Scale bar: 20 μm.

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