Fig. 3: RNA in situ hybridization for epithelial-mesenchymal transition-inducing transcription factors (EMT-TFs) in pleomorphic adenomas (PAs). | Laboratory Investigation

Fig. 3: RNA in situ hybridization for epithelial-mesenchymal transition-inducing transcription factors (EMT-TFs) in pleomorphic adenomas (PAs).

From: SLUG is a key regulator of epithelial-mesenchymal transition in pleomorphic adenoma

Fig. 3

A Representative hematoxylin and eosin image of a pleomorphic adenoma exhibiting the transition of epithelial cells to spindled mesenchymal cells and RNA in situ hybridization for SLUG, SNAIL, TWIST1, and ZEB1. B The Histo-scores (H-scores) of the four EMT-TFs in PAs were determined (n = 28). SLUG H-scores were much higher than the scores of other EMT-TFs. The data are shown as the mean ± SD. C Immunohistochemistry for P63 and calponin 1 identified neoplastic myoepithelial cells in PAs. The scatter plot shows a slight positive correlation between the H-scores of SLUG and proportion of P63-positive cells in PAs. Scale bar: 100 μm. ****P < 0.0001.

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