Fig. 5: Combined RNA in situ hybridization for SLUG and multiple immunohistochemistry for CK19 and P63 in pleomorphic adenomas.
From: SLUG is a key regulator of epithelial-mesenchymal transition in pleomorphic adenoma
A Representative hematoxylin and eosin image of a pleomorphic adenoma. B In situ hybridization for SLUG (white dots) and immunohistochemistry for CK19 (green cytoplasmic stain) and P63 (red nuclear stain) were performed on a single slide. The white boxed area is enlarged in (C), and the red boxed area is enlarged in (D). C P63-positive neoplastic myoepithelial cells exhibited strong SLUG expression (yellow arrows), whereas CK19-positive luminal cells did not express SLUG (white arrows). D P63-positive neoplastic myoepithelial cells tended to detach from the glands and transform into spindled stromal cells, expressing higher levels of SLUG (yellow arrows). Scale bar: 50 μm.
