Fig. 7: SLUG and PLAG1 expression in pleomorphic adenomas (PAs).
From: SLUG is a key regulator of epithelial-mesenchymal transition in pleomorphic adenoma
A, B Real-time PCR revealed a higher expression of PLAG1 in PAs (n = 22) compared with normal tissues (n = 10). There was no correlation between SLUG and PLAG1 mRNA expression. C Representative hematoxylin and eosin image of a pleomorphic adenoma expressing PLAG1 and SLUG. D Primary cultured PA cells exhibited characteristic histological features of PAs (epithelial cell clusters and surrounding spindle cells). E PLAG1 expression was downregulated by transfecting PA cells with PLAG1-siRNA; however, no significant change was detected in SLUG expression. F PLAG1 overexpression was induced by transfecting H293T cells with the pCMV6-PLAG1 vector; however, no change was observed in SLUG expression. **P < 0.01. ns, not significant.
