Fig. 2: IL-34 and PTPRZ gene deletion exacerbates K/BxN serum-transfer arthritis. | Laboratory Investigation

Fig. 2: IL-34 and PTPRZ gene deletion exacerbates K/BxN serum-transfer arthritis.

From: IL-34 and protein-tyrosine phosphatase receptor type-zeta-dependent mechanisms limit arthritis in mice

Fig. 2

A Experimental scheme. B Representative pictures of IL-34 KO, PTPRZ KO and WT hind limbs in arthritic and untreated normal WT (scale bar = 0.5 cm). C Histopathology score assessed in arthritic IL-34 KO and WT cross sectioned joints (4 μm, n = 20-24 joints/group [5-6 mice/group]). D Representative IL-34 KO and WT arthritic joint H&E sections (day 10, scale bar = 200 µm). E Histopathology score assessed in arthritic PTPRZ KO and WT cross-sections (4 μm, n = 20 joints/group [five mice/group]). F Representative PTPRZ KO and WT arthritic joint H&E sections (day 10, scale bar = 100 µm). For panels (D) and (F): arrows, infiltrates in the joint space (exudate); brackets, articular and adjacent tissue inflammation; BM bone marrow, C cartilage, Inf infiltrates, S synovium, Ti tibia, and TA talus. G Bone erosion evaluated using micro-CT scans (n = 6–8/group). Photos: Scale bar = 0.5 mm, white arrows, erosions on each of the ulnar epiphyses. Data are means ± SEM. Two-tailed Mann–Whitney U test (C, D) or Kruskal–Wallis test with a Dunn post-test (E). *P < 0.05. **P < 0.01. ***P < 0.001. ****P < 0.0001.

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