Fig. 5: Clearance of apoptotic cells is suppressed in IL-34 KO during peritonitis.
A Experimental scheme. B Number and frequency of Mø in the peritoneal fluid (PF) after inducing peritonitis (48, 72, and 96 h) (flow cytometry, n = 5/group). Representative of two experiments. C Experimental scheme: CSFE-labeled apoptotic neutrophils (2.5 × 106) generated from IL-34 KO and WT donors were injected (i.p.) into IL-34 KO and WT recipient mice 48 h after inducing peritonitis. Remaining CFSE+ cells were analyzed 3 h later by flow cytometry. D Number and frequency of CFSE-labeled neutrophils in the PF (flow cytometry, n = 5–8/group). E Normalized ratio of apoptotic neutrophils cleared by Mø in PF (averaged # remaining apoptotic neutrophils in B6 mice/ remaining apoptotic neutrophils, divided by # Mø in each sample/ averaged # Mø in B6 mice). F Experimental scheme for the clearance of apoptotic neutrophils (CSFE-labeled, IL-34 KO donors, 2.5 × 106 cells) analyzed as in (C). G Number and frequency of CFSE-labeled neutrophils in PF (flow cytometry, n = 5/group). H Normalized ratio of the number of apoptotic neutrophils cleared by Mø (calculated as in E). Data are represented as the means ± SEM. #P < 0.09. +P < 0.08. *P < 0.05. **P < 0.01; 2-tailed, Mann–Whitney U test.
