Table 1 Primer sequences for real-time PCR.
Gene | Forward primer | Reverse primer |
|---|---|---|
ASIC1a | 5′-GAGAAGCACAAGGAGATGACACG-3′ | 5′-AGACAAAGGGCACCAGGATAGG-3′ |
β-actin | 5′-CCCATCTATGAGGGTTACGC-3′ | 5′-TTTAATGTCACGCACGATTTC-3′ |
IL-1β | 5′-CTCAACTGTGAAATAGCAGCTTTC-3′ | 5′-GGACAGCCCAAGTCAAGG–3′ |
IL-6 | 5′-GAGCCCACCAGGAACGAAAGTC-3′ | 5′-TGTTGTGGGTGGTATCCTCTGTGAA-3′ |
TNF-α | 5′-ACTCCCAGAAAAGCAAGCAA-3′ | 5′-CAGTTCCACATCTCGGATCA-3′ |
