Fig. 1: L-Arginine supplementation fails to improve recovery from hindlimb ischemia. | Laboratory Investigation

Fig. 1: L-Arginine supplementation fails to improve recovery from hindlimb ischemia.

From: Increasing nitric oxide bioavailability fails to improve collateral vessel formation in humanized sickle cell mice

Fig. 1

A Arginase activity levels in serum and (B) non-ischemic (NIL) or ischemic (IL) hindlimb 7 days following hindlimb ischemia (HLI) surgery in sickle cell mice (SS) and their wildtype littermates (AA), n = 4, **P < 0.01, student’s unpaired t-test. C Serum arginine. D GABR. E Nitrate/nitrite (NOx) in AA and SS mice treated with L-Arginine (5% in drinking water) for 7 days, n = 4–8, **P < 0.01, One-way ANOVA, Sidak’s post hoc test. F Hydrogen peroxide (H2O2). G Peroxynitrite (ONOO-) levels in the ischemic hindlimbs or AA and SS mice treated with L-Arginine 7 days following HLI, n = 7, *P < 0.05, ***P < 0.001, One-way ANOVA, Sidak’s post hoc test. H Necrosis score and (I) perfusion recovery (IL/NIL perfusion) in AA and SS mice treated with L-Arginine 28 days following HLI, n = 4–8, *P < 0.05, ***P < 0.001, One-way ANOVA, Sidak’s post hoc test. J Representative LDPI image showing perfusion recovery in mice 28 days following HLI. IL area indicated with red box. Results expressed as mean ± SEM.

Back to article page