Fig. 2: Analysis of arginine pathways in sickle cell mice. | Laboratory Investigation

Fig. 2: Analysis of arginine pathways in sickle cell mice.

From: Increasing nitric oxide bioavailability fails to improve collateral vessel formation in humanized sickle cell mice

Fig. 2

A Serum citrulline and (B) ornithine levels in sickle cell mice (SS) and their wildtype littermates (AA). C Representative western blots for kidney expression of argininosuccinate synthase 1 (ASS1), argininosuccinate lyase (ASL), D Ornithine decarboxylase (ODC), ornthine transcarbamylase (OTC) and housekeeping protein β-actin in AA and SS mice with wildtype liver used as a positive control (+). E Densitometry results for ASS1. F ASL. G ODC. H OTC, n = 6–8, *P < 0.05, students unparied t-test. Results expressed as mean ± SEM. I Schematic depicting enzymes and metabolites involved in arginine synthesis and cycling.

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