Fig. 3: PIK3CD knockout inhibits glioma cell migration, invasion and xenograft tumor formation. | Laboratory Investigation

Fig. 3: PIK3CD knockout inhibits glioma cell migration, invasion and xenograft tumor formation.

From: Oncogenic potential of PIK3CD in glioblastoma is exerted through cytoskeletal proteins PAK3 and PLEK2

Fig. 3

The migration (A) and invasion (B) abilities of PIK3CD knockout clones were much reduced compared with parental U87-MG cells. Representative results from at least three independent experiments are shown (Mean ± SD, *p < 0.05). Magnification of the photomicrographs (x100). C The in vitro colony formation ability of the PIK3CD knockout cells was significantly impaired (Mean ± SD, *p < 0.05). D Orthotopic implantation showing xenograft tumor formation was inhibited in mice injected with the PIK3CD knockout cells. Arrows indicate the tumor formed in mouse brain injected with U87-MG. H&E histology confirmed the inability of PIK3CD knockout cells to form any tumor in the mouse brain.

Back to article page