Fig. 2: Spatial distribution of specific m/z species.

By matching the FFPE-HE staining A with the MSI measurement, specific tumor tissue areas can be visualized. The metabolite m/z 148.8547 is exclusively abundant in the tumor stroma B, whereas m/z 152.9452 is clearly overrepresented in tumor cell areas C. The overlay of the two visualizations D shows that the metabolites do not overlap in any area.