Fig. 1

BATF and BATF3 DNA binding, co-immunoprecipitation and expression in ALCL. a Left, EMSA of AP-1 TPA responsive element (TRE) complexes without (-) or with addition of specific antibodies, or isotype control (IC). Positions of AP-1 complexes, supershifts (ss) and a nonspecific band (n.s.) are indicated. Right, EMSA of IRF/AP-1 DNA binding at AICEs (AICE_Bcl11b; AICE; AICE_IL12RB). Underlined, IRF motif; bold, AP-1 motif; gray, intervening bases. The free probe of one representative EMSA is shown. b Left, EMSA of AICE_Bcl11b, performed as in a. Right, JUNB and BATF co-immunoprecipitations with anti-JUNB (top), anti-BATF (bottom) or isotype controls (IC). (Co-)immunoprecipitated proteins were detected by immunoblotting (WB). β-Actin and input were controls. c Left, BATF and BATF3 were analyzed at mRNA levels by RT-PCR (top) and at protein level by immunoblotting of nuclear extracts (bottom). GAPDH and PARP1 were controls. Right, BATF and BATF3 IHC of primary lymphomas. Top, BATF IHC of an ALK⁺ ALCL a, an ALK^– ALCL b and a mantle cell lymphoma [MCL; c]. Bottom, BATF3 IHC of an ALK⁺ ALCL d, an ALK^– ALCL e and a DLBCL f