Fig. 3 | Leukemia

Fig. 3

From: The AP-1-BATF and -BATF3 module is essential for growth, survival and TH17/ILC3 skewing of anaplastic large cell lymphoma

Fig. 3

Inhibition of global AP-1 activity by its dominant-negative A-Fos in FE-PD cells. Inducible A-Fos expression abrogates constitutive AP-1. Left, analysis of enriched GFP-positive cells by WB for A-Fos expression (top; FLAG antibody) and EMSA for DNA binding to TRE site (center, top) and BCL_11b AICE (center, bottom) after Dox addition for the indicated times. β-Actin and Sp1 DNA binding were controls. Two independent experiments (#1 and #2) are shown. Right, percentage of viable cells following Dox induction measured by PI staining and flow cytometry over time. ***P < 0.001

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