Fig. 2
From: The subclonal complexity of STIL-TAL1+ T-cell acute lymphoblastic leukaemia
Multicolour FISH in STIL-TAL1 T-ALL. Examples for evolutionary trees for samples CF5 and CF6 are shown. Percentages represent the frequency of each subclone as assessed by single-cell multicolour FISH with circle size representing relative frequencies. Photos shown are from sample CF5 using the following FISH probes: STIL-TAL1 (labelled spectrum red-spectrum green); p16.1 as a marker of CDKN2A loss (labelled Cy3, coloured blue in diagram); PTEN (labelled Biotin-Cy5, coloured pink in diagram). Note the STIL-TAL1 FISH probe is a subdeletion probe. Normal cells have two co-localised red-green signals. Cells with the STIL-TAL1 fusion due to 1p33 deletion lose one red signal while retaining the control green signal. ST F: STIL-TAL1 Fusion. Numbers (0, 1, 2) next to gene name (PTEN or CDKN2A) represent copy number results by FISH, e.g., 2 PTEN, two copies of PTEN, 0 CDKN2A, zero copies of CDKN2A
