Fig. 2
From: Kinase domain activation through gene rearrangement in multiple myeloma
EML4-ALK rearrangement in myeloma results in increased expression of the tyrosine kinase domain. EML4 and ALK are in opposite transcriptional orientation on chromosome 2. An inversion results in the active promoter from EML4 being placed upstream of the 5′ end of ALK, resulting in expression of the EML4-ALK fusion protein containing a tyrosine kinase domain. The fusion gene was confirmed by RT-PCR and Sanger sequencing. ALK is not expressed in adult tissue but EML4 is, however, in this sample, expression of the EML4-ALK fusion transcript was detected using 3′ expression array analysis
