Fig. 4
From: Kinase domain activation through gene rearrangement in multiple myeloma
Kinase gene fusions can be both clonal or subclonal. Clonality of fusion gene breakpoints was determined using droplet digital PCR (ddPCR) with probes specific for fusion gene breakpoints, unarranged alleles, or IGH translocation breakpoints. The EML4-ALK fusion was clonal being present in ~50% of the DNA molecules, equivalent to one allele having the rearrangement. ARHGAP27-MAP3K14 fusion was subclonal (10% of DNA, 20% of cells), as were KANK1-BRAF (15%/30%; 16%/32% after adjusting for purity) and GTF2I-BRAF (13%/26%; 19%/38% after adjusting for purity)
