Fig. 3 | Leukemia

Fig. 3

From: Exome sequencing of the TCL1 mouse model for CLL reveals genetic heterogeneity and dynamics during disease development

Fig. 3

Disease characteristics of TCL1 mice. Infiltration of mesenteric lymph nodes (a) and liver (b) of TCL1 mice C25 and D22 with CD5/CD19 positive CLL cells. FACS plots show CD5/CD19 stains of cell suspensions of the respective organ pregated for lymphocytes. c Spleen size variability shown by two spleens from mouse 347 and D22. d Skewing towards Vβ7+DN T cells in TCL1 mouse E31. FACS plots show CD4/CD8 distribution in CD3+ cells (left) and Vβ7 usage in DN CD3+ cells (right). e Sanger sequencing of Traf3 on DNA from the respective sorted splenocyte subset and germline (CD5CD19 negative hepatocytes) control from E31. Insertion (Ins) of leucine (L) at position 477 is indicated in blue. Protein domains of TRAF3 are depicted on top (adapted from Xie [36]. f Immunoblots from lysates of leukemic cells from CLL line C25 (Traf3 unmutated) and line E31 (Traf3 mutated) upon stimulation with the respective agents. Full length (~62 kD) and a shorter splice variant (~55 kD) are marked with arrows. (rm: recombinant mouse; p- means phosphorylated protein). (See also Supplement Figure S1 for detailed information)

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