Fig. 1

Phosphorylation of cortactin is high in ROR1^Pos CLL. a Immunoblot analysis of lysates prepared from primary ROR1^Pos or ROR1^Neg CLL cells of different patients; filters were probed with anti-cortactin, anti-phospho-cortactin (anti-pCortactin (Y421)), or anti-ROR1 antibody, as indicated on the left. The numbers above the top lane are ratios of band integrated optical density (IOD) of phosphorylated versus total cortactin. b Phosphorylation of cortactin (at Y421) was assessed by immunoblot analysis of lysates prepared from primary CLL cells of different patients with CLL cells that did (ROR1^Pos (n = 13)) or did not (ROR1^Neg (n = 11)) express ROR1. The ratios of band IOD of phosphorylated versus total cortactin were determined and plotted in the graph. Data are shown as mean ± SD. P < 0.001, as assessed by two-tailed Student’s t-test