Fig. 1
HRS cell lines display a global activation of long terminal repeat elements. a Sequence of the 82 base 5′ terminal region of the consensus THE1B transcript, plus the regions corresponding to the 5′ RACE primer region in the MaLR family member amplified by the primer, together with the overall THE1 consensus sequence. b RT-qPCR gene expression analysis measuring LTR-driven CSF1R expression (normalised to GAPDH expression). n = 3. Up-regulation in HRS cell lines P < 0.05, paired Student's t-test. c Alignment of RACE-Seq fragments to the human genome (hg19). Regions identified as RACE-Seq peaks are shown as black bars under fragment alignments. d Annotations of RACE-Seq peaks with the Repeat Masker hg19 repeat family annotation. e Overlap of active LTRs detected by RACE-Seq in 3 HRS cell lines (L428, L1236, and KM-H2) (green) and two control cell lines (Reh and Namalwa) (red). f Overlap of active LTRs detected by RACE-Seq in three cHL cell lines. g Clustering based on DICE index scores calculated for each pair of cell lines based on active LTR presence identified by RACE-Seq
